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  • br The DNA of all the

    2020-08-18


    The DNA of all the three (MCF-7, HCT-116, and MG-63) cancer ABT-263 treated with IC50 concentration, were extracted to confirm the apop-tosis by the shrinkage of treated cells [31]. The extracted DNA of the treated cells were run on the gel electrophoresis along with control DNA ladder of 1Kb. Results shown that there was high DNA fragmen-tation of the treated cells when compared with normal DNA ladder as shown in Fig. 12. Hence, our findings almost agree with the previous studies that cAgNPs with bovine serum albumin enhances the antic-ancer activity and also showed a good carrier for the therapeutic drugs to the target site [16,17,26,31]. The reason behind the conjugation of bovine serum albumin (BSA) with AgNPs was as follows: (1) the BSA is widely used as drug delivery, (2) nanocarrier development due to its excellent biocompatible properties with abundant hydrophobic binding sites, and (3) functional groups on the surface which easily ligate other molecules [32]. Moreover, the BSA is a serum protein which carries the nanoparticles, these serum proteins are found in the blood which is responsible for maintaining the pH of blood and works well under pH 7 to 9. The BSA is nontoxic and also immune system will not activate towards it. The BSA is function as a protein carrier and having the capability of binding both endogenous and exogenous drugs having multifunction binding surface sites with other target molecules.
    4. Conclusion
    Capped silver nanoparticles (cAgNPs) showed excellent anticancer against MCF-7, HCT-116, and MG-63 cancer lines as compared to AgNPs. These cAgNPs showed less toxicity towards normal 3T3 skin fibroblast cells. The cAgNPs also showed the excessive release of LDH which confirms the increase in the apoptosis of the cancer cells by using the IC50 concentration on dose-dependent manner. The DNA analysis showed complete fragmentation of treat cells when compared with a marker on an agarose gel. Hence these cAgNPs could be used as an effective antitumor agent but needs further cytotoxic studies and target drug delivery to avoid the toxicity on normal cells.
    Acknowledgment
    The author would like to thank the Centre of Research and Innovative Universiti Kuala Lumpur for providing financial assistance to carry out Helper virus study.
    Conflict of interest
    No conflict of interest among authors.
    References
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    Contents lists available at ScienceDirect
    Materials Science & Engineering C
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